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Irina S. Ryzhkina, Svetlana Yu. Sergeeva, Larisa A. Kostina, Lyaisan I. Murtazina, Andrei M. Petrov, Sergey A. Ryzhkin, Michail Ya. Melnikov
Diluted aqueous DNA solutions:
physicochemical and biological properties
Abstract
Abstract.
Aqueous dilute solutions of
salmon sperm DNA (DNA-ss), a readily available fishing industry material with
therapeutic and prophylactic properties, using a complex of physicochemical
methods (dynamic and electrophoretic light scattering, UV spectroscopy,
fluorescence, and redox potential) and ecotoxicological testing were studied. A
relationship between the percentage content of the dispersed phase hundreds of
nanometers in size, non-monotonic changes in redox potential and fluorescence
intensity, and the effect on the abundance of unicellular algae and the growth
of wheat seed roots was established. The most pronounced inhibitory effect of
DNA-ss solutions on plant biotest objects occurs in the dilution ranges at
which the maximum percentage of nanoassociates and an increase in redox
potential and fluorescence intensity are observed. Diluted DNA-ss solutions to
have a non-monotonic harmful effect on lower cladocerans are shown. The highest
mortality of cladocerans was observed at dilutions where an increase in the
percentage of structures thousands of nanometers in size and an increase in
fluorescence intensity in the λem 300 nm region were observed.
Key words:
dilute aqueous solutions,
salmon sperm DNA, self-organization, dispersed phase, UV absorption spectra,
fluorescence, hydrobionts
Copyright (C) Chemistry Dept., Moscow State University, 2002
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