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Tatiana A. Bogush1, Anna N. Grishanina1, Surena S. Dorzhieva1, Elena A. Bogush2, Alexander
M. Scherbakov1, 3, Vladislav Yu. Kirsanov2, Ekaterina N. Kosobokova1, Mikhail Ya. Melnikov4
Comprehensive immunophenotypic
characterization of sarcoma cell cultures by five tumor
molecular markers
Abstract
Abstract.Comprehensive immunophenotyping of sarcoma cell cultures was
performed for the expression of tumor markers associated with key biological
characteristics of malignant neoplasms: TUBB3, EMT/MET, PD-L1, ERα, and ERβ. Two pairs of cell cultures
with differences in molecular phenotype were formed: metastatic Mg-63 vs.
primary MNNG-Hos osteosarcoma and osteosarcoma Mg-63 vs fibrosarcoma HT-1080.
These pairs may be useful for assessing the contribution of the studied markers
to various biological characteristics of tumor cells and studying the
interactions between their regulatory mechanisms in mesenchymal tumors.
Key words:cell cultures, comprehensive
immunophenotyping, osteosarcoma Mg-63, osteosarcoma MNNG-Hos, fibrosarcoma
HT-1080, tumor markers TUBB3, EMT/MET, PD-L1, ERα,
ERβ
Copyright (C) Chemistry Dept., Moscow State University, 2002
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