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Danila A. Karpeev, Andrey V. Pirogov
Diethyl-2-(4-nitrobenzyliden)malonate synthesis and it’s
application for 2-methylmercaptoacetate detection by HPLC method
Abstract
Abstract. The basic requirements for an
effective reagent in the pre-column derivatisation of organic substances with
no chromophores are outlined. The study examined nitrofluorostyrenes,
dichlorostyrenes, and diethyl-2-(4-nitrobenzylidene)malonate as potential new
reagents. The latter was selected as the optimal reagent. The derivatisation of
2-methylmercaptoacetate as a test analyte was performed under mild conditions
(20 minutes at room temperature). Complete reaction with formation of a single
product was observed. The detection limit of 2-methylmercaptoacetate decreased
67-fold (to 15 ng/mL) compared to the initial limit.
Key words: HPLC,
derivatising reagents, nitrofluorostyrenes,
diethyl-2-(4-nitrobenzylidene)malonate, Michael reaction
Copyright (C) Chemistry Dept., Moscow State University, 2002
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