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Nikolay V. Panin, Dorel
T. Guranda, Irina V. Shapovalova, Vytas K. Švedas
Penicillin
acylase: a retrospective of studying the kinetics and thermodynamics of
practically significant reactions
Abstract
Abstract.The review considers
the contribution of works carried out at the scientific school of Ilya
Vasilievich Berezin to the study of the kinetics and thermodynamics of penicillin
acylase-catalyzed reactions. Methods for determining the activity of penicillin acylases,
the reversibility of the enzymatic hydrolysis of a number of penicillins,
cephalosporins and related compounds, the influence of the β-lactam ring
on the thermodynamics of the synthesis of new penicillins and cephalosporins by
direct condensation as well as by acyl transfer, the issues of optimizing the
conditions for enzymatic acyl transfer and the use of supersaturated reagent
solutions are discussed. The role of chromogenic substrates in the study of
penicillin acylase, the possibility of using the methods of titration of active
sites of the enzyme and the creation of “smart” biocatalysts based on
penicillin acylase due to the formation of conjugates with stimulus-sensitive
polymers are considered.
Key words: penicillin
acylase, kinetics of action, titration of enzyme active sites, chromogenic
substrates, study of difficult-to-detect reactions, beta-lactam antibiotics,
enzymatic modification, smart biocatalysts
Copyright (C) Chemistry Dept., Moscow State University, 2002
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