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Vitaly G. Grigorenko, Irina P. Andreeva, Elizaveta A. Melnichuk, Pavel
A. Levashov
Enzyme-linked immunosorbent assay of autoantibodies
against human β1-adrenergic receptor using recombinant antigens
Abstract
Abstract. E. coli strains have been created
as producers of recombinant β1-adreno-receptor epitopes as part of
chimeric proteins. The corresponding epitope sequences are located in the
C-terminal region of the human heart fatty acid binding protein (hH-FABP) and
separated from it by a linker sequence (Gly4Ser)3. A
solid-phase enzyme-linked immunosorbent assay (ELISA) for detection of
autoantibodies against β1-adrenergic receptor in human blood
serum based on a recombinant epitope has been developed. Blood sera of patients
(N = 76) with various diagnoses of cardiopathologies and other diseases
were analyzed. In some patients with a confirmed diagnosis of cardiovascular
diseases, a significantly increased level of autoantibodies to β1-adrenergic
receptor was detected, in most cases those with a diagnosis of acute myocardial
infarction.
Key words: enzyme immunoassay,
autoantibodies, β1-adrenergic receptor, fatty acid binding
protein, recombinant antigens, dilated cardiomyopathy
Copyright (C) Chemistry Dept., Moscow State University, 2002
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