|
Anna A. Filippova, Irina P. Andreeva, Galina V. Presnova, Mariya M. Ulyashova, Maya Yu. Rubtsova
Lateral flow hybridization assay for determination of
transcripts of TEM-type beta-lactamase genes in bacteria resistant to
antibiotics
Abstract
Abstract. A technique for lateral
flow hybridization analysis of specific bacterial beta-lactamase mRNAs has been
developed, including the preparation of a biotinylated target DNA and its
analysis on a test strip with immobilized oligonucleotide probes. When the DNA
target and probe structures are complementary, DNA duplexes are formed in the
test zone of the strip, which interact with the streptavidin conjugate with
gold nanoparticles. The method was used to determine the transcripts of the
TEM-type beta-lactamase genes isolated from the cultureof E. coli
– producers of the corresponding recombinant beta-lactamase. It has been shown
that single-stranded DNA target of 330 bases was revealed most effectively in
lateral flow analysis.
Key words: lateral flow analysis, beta-lactamases,
antibiotic resistance, mRNA, DNA
Copyright (C) Chemistry Dept., Moscow State University, 2002
|
|
